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Image Search Results
Journal: Scientific Reports
Article Title: Development of CpG-adjuvanted stable prefusion SARS-CoV-2 spike antigen as a subunit vaccine against COVID-19
doi: 10.1038/s41598-020-77077-z
Figure Lengend Snippet: Total anti-S IgG titers in mice immunized with S-2P with adjuvants . Sera from BALB/c mice in Fig. (N = 6 per group) immunized with 0, 1 or 5 μg of S-2P with CpG 1018, aluminum hydroxide or combination of both were quantified for the total amount of anti-S IgG with ELISA.
Article Snippet: For detection of IFN-γ, IL-2, IL-4, and IL-5, the culture supernatant from the 96-well microplates was harvested to analyze the levels of cytokines by ELISA using Mouse IFN-γ Quantikine ELISA Kit, Mouse IL-2 Quantikine ELISA Kit, Mouse IL-4 Quantikine ELISA Kit, and
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Scientific Reports
Article Title: Development of CpG-adjuvanted stable prefusion SARS-CoV-2 spike antigen as a subunit vaccine against COVID-19
doi: 10.1038/s41598-020-77077-z
Figure Lengend Snippet: Th1-dependent cytokine production induced by SARS-CoV-2 S-2P adjuvanted with CpG 1018, aluminum hydroxide, or CpG 1018 with aluminum hydroxide in mice. The antisera of BALB/c mice (N = 6 per group) were collected at 2 weeks after the second injection, the splenocytes were harvested and incubated with S-2P protein (2 μg), concanavalin A (0.1 μg; data not shown) for positive control, or complete RPMI 1640 medium only for negative control. After 20 h incubation, the levels of IFN-γ (left) and IL-2 (right) were analyzed by ELISA.
Article Snippet: For detection of IFN-γ, IL-2, IL-4, and IL-5, the culture supernatant from the 96-well microplates was harvested to analyze the levels of cytokines by ELISA using Mouse IFN-γ Quantikine ELISA Kit, Mouse IL-2 Quantikine ELISA Kit, Mouse IL-4 Quantikine ELISA Kit, and
Techniques: Injection, Incubation, Positive Control, Negative Control, Enzyme-linked Immunosorbent Assay
Journal: Scientific Reports
Article Title: Development of CpG-adjuvanted stable prefusion SARS-CoV-2 spike antigen as a subunit vaccine against COVID-19
doi: 10.1038/s41598-020-77077-z
Figure Lengend Snippet: Th2-dependent cytokine production induced by SARS-CoV-2 S-2P adjuvanted with CpG 1018, aluminum hydroxide, or CpG 1018/aluminum hydroxide in mice. The antisera of BALB/c mice (N = 6 per group) were collected at 2 weeks after the second injection, the splenocytes were harvested and stimulated as in Fig. . After 20 h incubation, the levels of IL-4 (left), IL-5 (middle), and IL-6 (right) released from the splenocytes were analyzed. For detection of cytokines, the culture supernatant was harvested to analyze the levels of cytokines by ELISA.
Article Snippet: For detection of IFN-γ, IL-2, IL-4, and IL-5, the culture supernatant from the 96-well microplates was harvested to analyze the levels of cytokines by ELISA using Mouse IFN-γ Quantikine ELISA Kit, Mouse IL-2 Quantikine ELISA Kit, Mouse IL-4 Quantikine ELISA Kit, and
Techniques: Injection, Incubation, Enzyme-linked Immunosorbent Assay